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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-4R Monoclonal antibody

IL-4R Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E4C11

Cat no : 67051-1-Ig

Synonyms

IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-4R Monoclonal antibody (67051-1-Ig)

    WB analysis using 67051-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MG-63 cells using IL-4R Monoclonal antibody (67051-1-Ig)

    WB analysis of MG-63 using 67051-1-Ig

    MG-63 cells were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IL-4R Monoclonal antibody (67051-1-Ig)

    WB analysis using 67051-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL4R antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA431 cells, Jurkat cells, MG-63 cells, SCaBER cells, HT-1376 cells, Raji cells, Ramos cells, Daudi cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67051-1-Ig targets IL-4R in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-4R fusion protein Ag28411 相同性解析による交差性が予測される生物種
Full Name interleukin 4 receptor
Calculated molecular weight 90 kDa
Observed molecular weight 120 kDa
GenBank accession numberNM_000418
Gene symbol IL-4R
Gene ID (NCBI) 3566
RRIDAB_2882364
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IL-4R antibody 67051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols