IL-4RA Monoclonal antibody
IL-4RA Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1E4C11
Cat no : 67051-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, Jurkat cells, MG-63 cells, SCaBER cells, HT-1376 cells, Raji cells, Ramos cells, Daudi cells, HUVEC cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67051-1-Ig targets IL-4RA in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | IL-4RA fusion protein Ag28411 相同性解析による交差性が予測される生物種 |
Full Name | interleukin 4 receptor |
Calculated molecular weight | 90 kDa |
Observed molecular weight | 120 kDa |
GenBank accession number | NM_000418 |
Gene symbol | IL-4R |
Gene ID (NCBI) | 3566 |
RRID | AB_2882364 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.
Protocols
Product Specific Protocols | |
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WB protocol for IL-4RA antibody 67051-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |