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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated IL-4RA Monoclonal antibody

IL-4RA Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4C11

Cat no : CL488-67051

Synonyms

IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated IL-4R Monoclonal ant (CL488-67051)

    IF Staining of human tonsillitis using CL488-67051

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite® Plus 488 IL-4R antibody (CL488-67051, Clone: 1E4C11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67051 targets IL-4RA in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-4RA fusion protein Ag28411 相同性解析による交差性が予測される生物種
Full Name interleukin 4 receptor
Calculated molecular weight 90 kDa
Observed molecular weight 120 kDa
GenBank accession numberNM_000418
Gene symbol IL-4R
Gene ID (NCBI) 3566
RRIDAB_2919413
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IL-4RA antibody CL488-67051Download protocol
Standard Protocols
Click here to view our Standard Protocols