☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

IMMT Polyclonal antibody

IMMT Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10179-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Mitofilin, MINOS2, MICOS complex subunit MIC60, MIC60, HMP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inCOLO 320 cells, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, mouse brain tissue, MCF-7 cells, rat brain tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10179-1-AP targets IMMT in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IMMT fusion protein Ag0102 相同性解析による交差性が予測される生物種
Full Name inner membrane protein, mitochondrial (mitofilin)
Calculated molecular weight 90 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC002412
Gene Symbol IMMT
Gene ID (NCBI) 10989
RRIDAB_2127193
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16891
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

Protocols

Product Specific Protocols
WB protocol for IMMT antibody 10179-1-APDownload protocol
IHC protocol for IMMT antibody 10179-1-APDownload protocol
IF protocol for IMMT antibody 10179-1-APDownload protocol
IP protocol for IMMT antibody 10179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nat Methods

Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models.

Authors - Mark Bates
humanWB

Cell Metab

Liver mitochondrial cristae organizing protein MIC19 promotes energy expenditure and pedestrian locomotion by altering nucleotide metabolism

Authors - Jee Hyung Sohn
mouseWB

Cell Metab

A cold-stress-inducible PERK/OGT axis controls TOM70-assisted mitochondrial protein import and cristae formation.

Authors - Pedro Latorre-Muro
WB

Cell Metab

The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake.

Authors - Carmelina Petrungaro
humanWB

Mol Cell

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

Authors - Yi Fu
humanWB

Mol Cell

The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation.

Authors - Katia Cosentino
Loading...