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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Mitofilin Polyclonal antib (CL594-10179)

    IF Staining of HeLa using CL594-10179

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Mitofilin antibody (CL594-10179) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite®594-conjugated Mitofilin Polyclonal antib (CL594-10179)

    FC experiment of HEK-293T using CL594-10179

    1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human Mitofilin (CL594-10179) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO Validated

CoraLite®594-conjugated IMMT Polyclonal antibody

IMMT Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-10179

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

Mitofilin, MINOS2, MICOS complex subunit MIC60, MIC60, HMP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10179 targets IMMT in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IMMT fusion protein Ag0102 相同性解析による交差性が予測される生物種
Full Name inner membrane protein, mitochondrial (mitofilin)
Calculated molecular weight 90 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC002412
Gene Symbol IMMT
Gene ID (NCBI) 10989
RRIDAB_2919722
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16891
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

Protocols

Product Specific Protocols
IF protocol for CL594 IMMT antibody CL594-10179Download protocol
FC protocol for CL594 IMMT antibody CL594-10179Download protocol
Standard Protocols
Click here to view our Standard Protocols