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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IMMT Monoclonal antibody

IMMT Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D2E8

Cat no : CL488-68226

Synonyms

DKFZp779P1653, HMP, IMMT, Mitofilin, P87, P87/89, P89, PIG4, PIG52

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated IMMT Monoclonal anti (CL488-68226)

    IF Staining of HeLa using CL488-68226

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 IMMT antibody (CL488-68226, Clone: 2D2E8 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68226 targets IMMT in IF/ICC applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IMMT fusion protein Ag0102 相同性解析による交差性が予測される生物種
Full Name inner membrane protein, mitochondrial (mitofilin)
Calculated molecular weight 90 kDa
Observed molecular weight90 kDa
GenBank accession numberBC002412
Gene symbol IMMT
Gene ID (NCBI) 10989
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IMMT antibody CL488-68226Download protocol
Standard Protocols
Click here to view our Standard Protocols