CoraLite®555-conjugated IMP3 Monoclonal antibody
IMP3 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
1E7D6
Cat no : CL555-66247
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-66247 targets IMP3 in FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IMP3 fusion protein Ag23602 相同性解析による交差性が予測される生物種 |
| Full Name | IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast) |
| Calculated molecular weight | 184 aa, 22 kDa |
| GenBank accession number | BC020557 |
| Gene symbol | IMP3 |
| Gene ID (NCBI) | 55272 |
| RRID | AB_2919680 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004].
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL555 IMP3 antibody CL555-66247 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
