IMPDH1 Recombinant antibody, PBS Only (Capture)

Raji cells were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84913-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84913-1-PBS in a different storage buffer formulation.

Sandwich ELISA standard curve of MP01726-1, Human IMPDH1 Recombinant Matched Antibody Pair - PBS only. 84913-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17473. 84913-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

The mean IMPDH1 concentration was determined to be 27.79 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load and 45.02 ng/mL in A431 cell extract based on a 1.30 mg/mL extract load.

Biolayer interferometry (BLl) kinetic assays of 84913-1-RR against Human IMPDH1 were performed. The affinity constant is 4.41 nM.