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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INA Monoclonal antibody (67120-1-Ig)

    WB analysis using 67120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using INA Monoclonal antibody (67120-1-Ig)

    WB analysis using 67120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using INA Monoclonal antibody (67120-1-Ig)

    IHC staining of human pancreas cancer using 67120-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67120-1-Ig (INA antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using INA Monoclonal antibody (67120-1-Ig)

    IHC staining of human pancreas cancer using 67120-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67120-1-Ig (INA antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using INA Monoclonal antibody (67120-1-Ig)

    IF Staining of SH-SY5Y using 67120-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using INA antibody (67120-1-Ig, Clone: 3C12A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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INA Monoclonal antibody

INA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67120-1-Ig
Clone No.3C12A8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected infetal human brain tissue, rat cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67120-1-Ig targets INA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen INA fusion protein Ag13655 相同性解析による交差性が予測される生物種
Full Name internexin neuronal intermediate filament protein, alpha
Calculated molecular weight 55 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC006359
Gene Symbol INA
Gene ID (NCBI) 9118
RRIDAB_2882423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16352
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INA, also named as Alpha internexin, is a 66 kDa intermediate filament protein which is involved in the morphogenesis of neurons. INA is mainly expressed in various kinds of central and peripheral neurons from early development and highly expressed in neurodegenerative and neuronal death cells. The MW of INA ranges from 60 kDa in human, pig and mouse to 66 kDa in rat.(PMID: 29940892, 2201753)

Protocols

Product Specific Protocols
WB protocol for INA antibody 67120-1-IgDownload protocol
IHC protocol for INA antibody 67120-1-IgDownload protocol
IF protocol for INA antibody 67120-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols