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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INA Monoclonal antibody, PBS Only

INA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C12A8

Cat no : 67120-1-PBS

Synonyms

Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INA Monoclonal antibody (67120-1-Ig)

    WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using INA Monoclonal antibody (67120-1-Ig)

    WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using INA Monoclonal antibody (67120-1-Ig)

    IHC staining of human pancreas cancer using 67120-1-Ig (same clone as 67120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67120-1-Ig (INA antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using INA Monoclonal antibody (67120-1-Ig)

    IHC staining of human pancreas cancer using 67120-1-Ig (same clone as 67120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67120-1-Ig (INA antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using INA Monoclonal antibody (67120-1-Ig)

    IF Staining of SH-SY5Y using 67120-1-Ig (same clone as 67120-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using INA antibody (67120-1-Ig, Clone: 3C12A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67120-1-PBS targets INA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen INA fusion protein Ag13655 相同性解析による交差性が予測される生物種
Full Name internexin neuronal intermediate filament protein, alpha
Calculated molecular weight 55 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC006359
Gene symbol INA
Gene ID (NCBI) 9118
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

INA, also named as Alpha internexin, is a 66 kDa intermediate filament protein which is involved in the morphogenesis of neurons. INA is mainly expressed in various kinds of central and peripheral neurons from early development and highly expressed in neurodegenerative and neuronal death cells. The MW of INA ranges from 60 kDa in human, pig and mouse to 66 kDa in rat.(PMID: 29940892, 2201753)