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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ING2 Polyclonal antibody (11560-1-AP)

    WB analysis of HEK-293 using 11560-1-AP (same clone as 11560-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using ING2 Polyclonal antibody (11560-1-AP)

    WB analysis of human liver using 11560-1-AP (same clone as 11560-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ING2 Polyclonal antibody (11560-1-AP)

    IP experiment of HEK-293 using 11560-1-AP (same clone as 11560-1-PBS)

    IP result of anti-ING2 (IP:11560-1-AP, 3ug; Detection:11560-1-AP 1:500) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ING2 Polyclonal antibody (11560-1-AP)

    IHC staining of human colon cancer using 11560-1-AP (same clone as 11560-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ING2 Polyclonal antibody (11560-1-AP)

    IHC staining of human colon cancer using 11560-1-AP (same clone as 11560-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ING2 Polyclonal antibody (11560-1-AP)

    IHC staining of human cervical cancer using 11560-1-AP (same clone as 11560-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ING2 Polyclonal antibody (11560-1-AP)

    IHC staining of human cervical cancer using 11560-1-AP (same clone as 11560-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ING2 Polyclonal antibody (11560-1-AP)

    IF Staining of HEK-293 using 11560-1-AP (same clone as 11560-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 11560-1-AP (ING2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11560-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ING2 Polyclonal antibody, PBS Only

ING2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11560-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11560-1-PBS targets ING2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ING2 fusion protein Ag2121 相同性解析による交差性が予測される生物種
Full Name inhibitor of growth family, member 2
Calculated molecular weight 280 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC030128
Gene Symbol ING2
Gene ID (NCBI) 3622
RRIDAB_2127771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H160
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. It is induced by the DNA-damaging agent neocarzinostatin. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program. Catalog#11560-1-AP is a rabbit polyclonal antibody raised against full length ING2 of human origin.