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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using ING2 Monoclonal antibody (66512-1-Ig)

    WB analysis of HSC-T6 using 66512-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ING2 Monoclonal antibody (66512-1-Ig)

    WB analysis of NIH/3T3 using 66512-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using ING2 Monoclonal antibody (66512-1-Ig)

    WB analysis of T-47D using 66512-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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ING2 Monoclonal antibody

ING2 Monoclonal Antibody for WB, ELISA
Cat No. 66512-1-Ig
Clone No.2C3A7

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, ELISA

Synonyms

ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHSCT6 cells, T-47D cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66512-1-Ig targets ING2 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ING2 fusion protein Ag19291 相同性解析による交差性が予測される生物種
Full Name inhibitor of growth family, member 2
Calculated molecular weight 280 aa, 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC030128
Gene Symbol ING2
Gene ID (NCBI) 3622
RRIDAB_2881875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H160
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program.

Protocols

Product Specific Protocols
WB protocol for ING2 antibody 66512-1-IgDownload protocol
FC protocol for ING2 antibody 66512-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols