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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using ING2 Monoclonal antibody (66512-1-Ig)

    WB analysis of HSC-T6 using 66512-1-Ig (same clone as 66512-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66512-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using ING2 Monoclonal antibody (66512-1-Ig)

    WB analysis of NIH/3T3 using 66512-1-Ig (same clone as 66512-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66512-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using ING2 Monoclonal antibody (66512-1-Ig)

    WB analysis of T-47D using 66512-1-Ig (same clone as 66512-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66512-1-Ig (ING2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66512-1-PBS in a different storage buffer formulation.

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ING2 Monoclonal antibody, PBS Only

ING2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66512-1-PBS
Clone No.2C3A7

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, Indirect ELISA

Synonyms

ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66512-1-PBS targets ING2 in WB, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ING2 fusion protein Ag19291 相同性解析による交差性が予測される生物種
Full Name inhibitor of growth family, member 2
Calculated molecular weight 280 aa, 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC030128
Gene Symbol ING2
Gene ID (NCBI) 3622
RRIDAB_2881875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H160
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program.