• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INO80 Monoclonal antibody

INO80 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F11C5

Cat no : 68064-1-Ig

Synonyms

hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using INO80 Monoclonal antibody (68064-1-Ig)

    WB analysis using 68064-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using INO80 Monoclonal antibody (68064-1-Ig)

    WB analysis using 68064-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using INO80 Monoclonal antibody (68064-1-Ig)

    IHC staining of human colon cancer using 68064-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using INO80 Monoclonal antibody (68064-1-Ig)

    IHC staining of human colon cancer using 68064-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of PC-12 cells using INO80 Monoclonal antibody (68064-1-Ig)

    FC experiment of PC-12 using 68064-1-Ig

    1X10^6 PC-12 cells were intracellularly stained with 0.8 ug Anti-Human INO80 (68064-1-Ig, Clone:1F11C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68064-1-Ig targets INO80 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen INO80 fusion protein Ag20265 相同性解析による交差性が予測される生物種
Full Name INO80 homolog (S. cerevisiae)
Calculated molecular weight 1556 aa, 177 kDa
Observed molecular weight177 kDa
GenBank accession numberBC146785
Gene symbol INO80
Gene ID (NCBI) 54617
RRIDAB_2918805
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.

Protocols

Product Specific Protocols
WB protocol for INO80 antibody 68064-1-IgDownload protocol
IHC protocol for INO80 antibody 68064-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols