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Validation Data Gallery

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  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite® Plus 488-conjugated INO80 Monoclonal ant (CL488-68064)

    FC experiment of PC-12 using CL488-68064

    1X10^6 PC-12 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human INO80 (CL488-68064, Clone:1F11C5) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated INO80 Monoclonal antibody

INO80 Monoclonal Antibody for FC (Intra)
Cat No. CL488-68064
Clone No.1F11C5

Host / Isotype

Mouse / IgG1

Reactivity

rat

Applications

FC (Intra)

Synonyms

hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inPC-12 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68064 targets INO80 in FC (Intra) applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen INO80 fusion protein Ag20265 相同性解析による交差性が予測される生物種
Full Name INO80 homolog (S. cerevisiae)
Calculated molecular weight 1556 aa, 177 kDa
Observed molecular weight177 kDa
GenBank accession numberBC146785
Gene Symbol INO80
Gene ID (NCBI) 54617
RRIDAB_2934618
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9ULG1
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 INO80 antibody CL488-68064Download protocol
Standard Protocols
Click here to view our Standard Protocols