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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INTS9 Polyclonal antibody (11657-1-AP)

    WB analysis using 11657-1-AP (same clone as 11657-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11657-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using INTS9 Polyclonal antibody (11657-1-AP)

    IP experiment of HeLa using 11657-1-AP (same clone as 11657-1-PBS)

    IP result of anti-INTS9 (IP:11657-1-AP, 4ug; Detection:11657-1-AP 1:1000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 11657-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using INTS9 Polyclonal antibody (11657-1-AP)

    IHC staining of human stomach using 11657-1-AP (same clone as 11657-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11657-1-AP (INTS9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11657-1-PBS in a different storage buffer formulation.

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INTS9 Polyclonal antibody, PBS Only

INTS9 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11657-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

RC 74, INTS 9, Int9, CPSF2L, CPSF2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11657-1-PBS targets INTS9 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INTS9 fusion protein Ag2226 相同性解析による交差性が予測される生物種
Full Name integrator complex subunit 9
Calculated molecular weight 658 aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC025267
Gene Symbol INTS9
Gene ID (NCBI) 55756
RRIDAB_2127514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NV88
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

INTS9, also known as Integrator Complex Subunit 9, is part of the core cleavage module consisting of INTS4/INTS9/INTS11 involved in processing small nuclear RNA (snRNA) molecules. It is a subunit of the Integrator complex, which comprises 15 subunits (including INTS6L), and plays a crucial role in the 3' end processing of snRNAs. By participating in the biogenesis of snRNAs, INTS9 contributes to the proper functioning of the spliceosome, a cellular machinery responsible for the accurate splicing of precursor messenger RNA (pre-mRNA) molecules. This process is essential for generating mature messenger RNA (mRNA), which is subsequently translated into functional proteins.