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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IPMK Polyclonal antibody (30161-1-AP)

    WB analysis using 30161-1-AP (same clone as 30161-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30161-1-AP (IPMK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30161-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IPMK Polyclonal antibody (30161-1-AP)

    IF Staining of A431 using 30161-1-AP (same clone as 30161-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using IPMK antibody (30161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30161-1-PBS in a different storage buffer formulation.

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IPMK Polyclonal antibody, PBS Only

IPMK Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30161-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30161-1-PBS targets IPMK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IPMK fusion protein Ag32377 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate multikinase
Observed molecular weight42-47 kDa
GenBank accession numberBC065709
Gene Symbol IPMK
Gene ID (NCBI) 253430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFU5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IPMK plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). It is required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6).