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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated IPO5 Monoclonal antibody

IPO5 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E2B9

Cat no : CL488-67962

Synonyms

IPO5, Karyopherin beta 3, Karyopherin beta-3, KPNB3, Ran binding protein 5, Ran-binding protein 5, RANBP5, 1E2B9, IMB3, Imp5, importin 5, Importin subunit beta 3, Importin subunit beta-3, Importin-5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated IPO5 Monoclonal anti (CL488-67962)

    FC experiment of A431 using CL488-67962

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IPO5 (CL488-67962, Clone:1E2B9) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67962 targets IPO5 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IPO5 fusion protein Ag30955 相同性解析による交差性が予測される生物種
Full Name importin 5
Observed molecular weight124 kDa
GenBank accession numberBC001497
Gene symbol IPO5
Gene ID (NCBI) 3843
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IPO5 is a member of the importin beta family. Structurally, the protein adopts the shape of a right-hand solenoid and is composed of 24 HEAT repeats. IPO5 facilitates cytoplasmic polyadenylation element-binding protein CPEB3 translocation by binding to the RRM1 motif of CPEB3 in neurons. NMDAR signaling increases RanBP1 expression and reduces the level of cytoplasmic GTP-bound Ran. These changes enhance CPEB3-IPO5 interaction, which consequently accelerates the nuclear import of CPEB3 and promotes its nuclear function.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IPO5 antibody CL488-67962Download protocol
Standard Protocols
Click here to view our Standard Protocols