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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRAK3 Recombinant antibody, PBS Only (Detector)

IRAK3 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240975C5

Cat no : 83876-2-PBS

Synonyms

IRAK 3, Interleukin-1 receptor-associated kinase 3, Inactive IL-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, ASRT5

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis of mouse lung using 83876-2-RR (same clone as 83876-2-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis using 83876-2-RR (same clone as 83876-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00849-2

    Cytometric bead array standard curve of MP00849-2

    Cytometric bead array standard curve of MP00849-2, IRAK3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83876-3-PBS. Detection antibody: 83876-2-PBS. Standard: Ag4959. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00849-3

    Cytometric bead array standard curve of MP00849-3

    Cytometric bead array standard curve of MP00849-3, IRAK3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83876-1-PBS. Detection antibody: 83876-2-PBS. Standard: Ag4959. Range: 0.625-80 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83876-2-PBS targets IRAK3 as part of a matched antibody pair:

MP00849-2: 83876-3-PBS capture and 83876-2-PBS detection (validated in Cytometric bead array)

MP00849-3: 83876-1-PBS capture and 83876-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IRAK3 fusion protein Ag4959 相同性解析による交差性が予測される生物種
Full Name interleukin-1 receptor-associated kinase 3
Calculated molecular weight 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC057800
Gene symbol IRAK3
Gene ID (NCBI) 11213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IRAK3 (Interleukin-1 receptor-associated kinase-3, also named IRAKM) as a kinase-deficient member of the TLR/IRAK family has been shown to negatively regulate Toll-like receptor (TLR)-mediated cell signaling (PMID: 33590831).