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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRAK3 Recombinant antibody

IRAK3 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240975C5

Cat no : 83876-2-RR

Synonyms

IRAK 3, Interleukin-1 receptor-associated kinase 3, Inactive IL-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, ASRT5

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis of THP-1 using 83876-2-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis of mouse lung using 83876-2-RR

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IRAK3 Recombinant antibody (83876-2-RR)

    WB analysis using 83876-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83876-2-RR is IRAK3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IRAK3 fusion protein Ag4959 相同性解析による交差性が予測される生物種
Full Name interleukin-1 receptor-associated kinase 3
Calculated molecular weight 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC057800
Gene symbol IRAK3
Gene ID (NCBI) 11213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRAK3 (Interleukin-1 receptor-associated kinase-3, also named IRAKM) as a kinase-deficient member of the TLR/IRAK family has been shown to negatively regulate Toll-like receptor (TLR)-mediated cell signaling (PMID: 33590831).

Protocols

Product Specific Protocols
WB protocol for IRAK3 antibody 83876-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols