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KD/KO Validated

IRF1 Polyclonal antibody

Name: IRF1 Polyclonal antibody
Brand: Proteintech
SKU: 11335-1-AP

IRF1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11335-1-AP

Synonyms

MAR, IRF-1, IRF 1, interferon regulatory factor 1

Validation Data Gallery

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AllWBIHCIF/ICCFC (Intra)

WB analysis of HL-60 using 11335-1-AP
WB result of IRF1 antibody (11335-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRF1 transfected HL-60 cells.
WB analysis using 11335-1-AP
Western blot analysis of HeLa cells, untreated or treated with Human IFN-γ (hIFN-γ) （HZ-1301） (100 ng/ml, 48 hr), using 11335-1-AP (IRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 11335-1-AP
IFN gamma treated THP-1 cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 11335-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11335-1-AP (IRF1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 11335-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11335-1-AP (IRF1 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 11335-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed IFN gamma treated HepG2 cells using IRF1 antibody (11335-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of Jurkat using 11335-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human IRF1 (11335-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Cat No. 11335-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	IFN gamma treated HeLa cells, HL-60 cells, IFN gamma treated THP-1 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	IFN gamma treated HepG2 cells
Positive FC (Intra) detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 37 publications below
IHC	See 7 publications below
IF	See 9 publications below
IP	See 2 publications below
CoIP	See 1 publications below
ChIP	See 3 publications below

Product Information

11335-1-AP targets IRF1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, pig, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IRF1 fusion protein Ag1879 相同性解析による交差性が予測される生物種
Full Name	IRF 1
Calculated molecular weight	325 aa, 37 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC009483
Gene symbol	IRF1
Gene ID (NCBI)	3659
RRID	AB_2877759
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

Protocols

Product Specific Protocols
WB protocol for IRF1 antibody 11335-1-AP	Download protocol
IHC protocol for IRF1 antibody 11335-1-AP	Download protocol
IF protocol for IRF1 antibody 11335-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Cancer Commun (Lond) Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies Authors - Lu Tang View Article
human	WB	J Immunother Cancer Mefloquine enhances the efficacy of anti-PD-1 immunotherapy via IFN-γ-STAT1-IRF1-LPCAT3-induced ferroptosis in tumors Authors - Qian Tao View Article
human	WB	J Immunother Cancer Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis. Authors - Weinan Guo View Article
mouse	WB,IF	Cell Commun Signal IRF1 regulation of ZBP1 links mitochondrial DNA and chondrocyte damage in osteoarthritis Authors - Kai Sun KD Validated View Article
human	WB,IHC,IF	Free Radic Biol Med IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis Authors - Yao Zhang KD Validated View Article
human	WB	Front Immunol Toxoplasma Effector TgIST Targets Host IDO1 to Antagonize the IFN-γ-Induced Anti-parasitic Response in Human Cells. Authors - Hironori Bando View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRF1 Polyclonal antibody

IRF1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis of HL-60 using 11335-1-AP

WB analysis using 11335-1-AP

WB analysis using 11335-1-AP

IHC staining of human breast cancer using 11335-1-AP

IHC staining of human breast cancer using 11335-1-AP

IF Staining of HepG2 using 11335-1-AP

FC experiment of Jurkat using 11335-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies

Mefloquine enhances the efficacy of anti-PD-1 immunotherapy via IFN-γ-STAT1-IRF1-LPCAT3-induced ferroptosis in tumors

Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.

IRF1 regulation of ZBP1 links mitochondrial DNA and chondrocyte damage in osteoarthritis

IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis

Toxoplasma Effector TgIST Targets Host IDO1 to Antagonize the IFN-γ-Induced Anti-parasitic Response in Human Cells.