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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IRF1 Polyclonal antibody

IRF1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11335

Synonyms

MAR, IRF-1, IRF 1, interferon regulatory factor 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated IRF1 Polyclonal anti (CL488-11335)

    IF Staining of HepG2 using CL488-11335

    Immunofluorescent analysis of (-20°C Ethanol) fixed IFN gamma treated HepG2 cells using CoraLite® Plus 488 IRF1 antibody (CL488-11335) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated IRF1 Polyclonal anti (CL488-11335)

    FC experiment of Jurkat using CL488-11335

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IRF1 (CL488-11335) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inIFN gamma treated HepG2 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11335 targets IRF1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF1 fusion protein Ag1879 相同性解析による交差性が予測される生物種
Full Name IRF 1
Calculated molecular weight 325 aa, 37 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC009483
Gene symbol IRF1
Gene ID (NCBI) 3659
RRIDAB_2919026
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IRF1 antibody CL488-11335Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Sci Adv

XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility

Authors - Ming Kuang
View Article