Validation Data Gallery
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Tested Applications
| Positive WB detected in | U266 cells, human spleen tissue, pig spleen tissue, rat spleen tissue, HuT 78 cells, MJ cells |
| Positive IHC detected in | human tonsillitis Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
66451-1-Ig targets IRF4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IRF4 fusion protein Ag25203 相同性解析による交差性が予測される生物種 |
| Full Name | IRF 4 |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 48-52 kDa |
| GenBank accession number | BC015752 |
| Gene Symbol | IRF4 |
| Gene ID (NCBI) | 3662 |
| ENSEMBL Gene ID | ENSG00000137265 |
| RRID | AB_2881820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15306 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IRF4 antibody 66451-1-Ig | Download protocol |
| IHC protocol for IRF4 antibody 66451-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioeng Transl Med Macrophage-targeted delivery of siRNA to silence Mecp2 gene expression attenuates pulmonary fibrosis. | ||
Biochim Biophys Acta Mol Cell Res RNF2 promotes the progression of colon cancer by regulating ubiquitination and degradation of IRF4 |