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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRF4 Monoclonal antibody, PBS Only (Detector)

IRF4 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12D5

Cat no : 66451-1-PBS

Synonyms

MUM1/IRF4, Lymphocyte-specific interferon regulatory factor, LSIRF, IRF-4, IRF 4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human spleen tissue using IRF4 Monoclonal antibody (66451-1-Ig)

    WB analysis of human spleen using 66451-1-Ig (same clone as 66451-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRF4 Monoclonal antibody (66451-1-Ig)

    WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IRF4 Monoclonal antibody (66451-1-Ig)

    WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig spleen tissue using IRF4 Monoclonal antibody (66451-1-Ig)

    WB analysis of pig spleen using 66451-1-Ig (same clone as 66451-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50012-1

    Sandwich ELISA standard curve of MP50012-1

    Sandwich ELISA standard curve ofMP50012-1, IRF4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66451-2-PBS. Detection antibody: HRP-conjugated 66451-1-PBS. Standard: Ag25203. Range: 0.02-20 ng/mL.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IRF4 Monoclonal antibody (66451-1-Ig)

    IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IRF4 Monoclonal antibody (66451-1-Ig)

    IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66451-1-PBS targets IRF4 as part of a matched antibody pair:

MP50012-1: 66451-2-PBS capture and 66451-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IRF4 fusion protein Ag25203 相同性解析による交差性が予測される生物種
Full Name IRF 4
Calculated molecular weight 52 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberBC015752
Gene symbol IRF4
Gene ID (NCBI) 3662
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.