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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRF5 Monoclonal antibody

IRF5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A4A10

Cat no : 66835-1-Ig

Synonyms

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using IRF5 Monoclonal antibody (66835-1-Ig)

    WB analysis of A549 using 66835-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using IRF5 Monoclonal antibody (66835-1-Ig)

    WB analysis of THP-1 using 66835-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using IRF5 Monoclonal antibody (66835-1-Ig)

    WB analysis of RAW 264.7 using 66835-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IRF5 Monoclonal antibody (66835-1-Ig)

    WB analysis of HeLa using 66835-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, THP-1 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66835-1-Ig targets IRF5 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IRF5 fusion protein Ag5442 相同性解析による交差性が予測される生物種
Full Name interferon regulatory factor 5
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC004201
Gene symbol IRF5
Gene ID (NCBI) 3663
RRIDAB_2882178
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). IRF5 is also used as M1 marcrophage lineage marker. Alternative splice variant encoding different isoforms exist.

Protocols

Product Specific Protocols
WB protocol for IRF5 antibody 66835-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols