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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IRF8 Monoclonal antibody (67142-1-Ig)

    WB analysis using 67142-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67142-1-Ig (IRF8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using IRF8 Monoclonal antibody (67142-1-Ig)

    IF Staining of THP-1 using 67142-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IRF8 antibody (67142-1-Ig, Clone: 3A10A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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IRF8 Monoclonal antibody

IRF8 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67142-1-Ig
Clone No.3A10A4

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

H ICSBP, ICSBP, ICSBP1, IRF 8, IRF8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 594
Size/Concentration:  150 μL, 1700 ug/ml
20 μL, 1700 ug/ml
150 μL, 1700 ug/ml

¥67,000/ 150 μL

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRamos cells, Raji cells, Daudi cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67142-1-Ig targets IRF8 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IRF8 fusion protein Ag19909 相同性解析による交差性が予測される生物種
Full Name ICSBP1
Calculated molecular weight 426 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC126247
Gene Symbol IRF8
Gene ID (NCBI) 3394
RRIDAB_2882441
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02556
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF8 also named as ICSBP1, is a transcription factor of the IFN regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)). It plays a negative regulatory role in cells of the immune system. It is predominantly in lymphoid tissues.

Protocols

Product Specific Protocols
WB protocol for IRF8 antibody 67142-1-IgDownload protocol
IF protocol for IRF8 antibody 67142-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols