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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 594-conjugated IRF8 Monoclonal antibody

IRF8 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

3A10A4

Cat no : CL594-67142

Synonyms

H ICSBP, ICSBP, ICSBP1, interferon regulatory factor 8, IRF 8, IRF8

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CoraLite® Plus 594-conjugated IRF8 Monoclonal anti (CL594-67142)

    IF Staining of THP-1 using CL594-67142

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 594 IRF8 antibody (CL594-67142, Clone: 3A10A4 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67142 targets IRF8 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IRF8 fusion protein Ag19909 相同性解析による交差性が予測される生物種
Full Name ICSBP1
Calculated molecular weight 426 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC126247
Gene symbol IRF8
Gene ID (NCBI) 3394
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission maxima wavelengths594 nm / 615 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF8 also named as ICSBP1, is a transcription factor of the IFN regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)). It plays a negative regulatory role in cells of the immune system. It is predominantly in lymphoid tissues.

Protocols

Product Specific Protocols
IF protocol for CL Plus 594 IRF8 antibody CL594-67142Download protocol
Standard Protocols
Click here to view our Standard Protocols