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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IRX6 Polyclonal antibody (24440-1-AP)

    WB analysis using 24440-1-AP (same clone as 24440-1-PBS)

    Mouse Heart tissue lysates were subjected to SDS PAGE followed by western blot with 24440-1-AP (IRX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IRX6 Polyclonal antibody (24440-1-AP)

    IHC staining of human colon cancer using 24440-1-AP (same clone as 24440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24440-1-AP (IRX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IRX6 Polyclonal antibody (24440-1-AP)

    IHC staining of human colon cancer using 24440-1-AP (same clone as 24440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24440-1-AP (IRX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using IRX6 Polyclonal antibody (24440-1-AP)

    IHC staining of mouse heart using 24440-1-AP (same clone as 24440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 24440-1-AP (IRX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using IRX6 Polyclonal antibody (24440-1-AP)

    IHC staining of mouse heart using 24440-1-AP (same clone as 24440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 24440-1-AP (IRX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24440-1-PBS in a different storage buffer formulation.

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IRX6 Polyclonal antibody, PBS Only

IRX6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24440-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24440-1-PBS targets IRX6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRX6 fusion protein Ag19623 相同性解析による交差性が予測される生物種
Full Name iroquois homeobox 6
Calculated molecular weight 446 aa, 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC136573
Gene Symbol IRX6
Gene ID (NCBI) 79190
RRIDAB_2918082
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78412
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IRX6 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.