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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ITCH Monoclonal antibody

ITCH Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E3G9

Cat no : CL488-67757

Synonyms

EC:2.3.2.26, E3 ubiquitin-protein ligase Itchy homolog, Atrophin-1-interacting protein 4, AIP4, AIF4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ITCH Monoclonal anti (CL488-67757)

    IF Staining of HepG2 using CL488-67757

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ITCH antibody (CL488-67757, Clone: 2E3G9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated ITCH Monoclonal anti (CL488-67757)

    FC experiment of HepG2 using CL488-67757

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ITCH (CL488-67757, Clone:2E3G9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67757 targets ITCH in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ITCH fusion protein Ag14688 相同性解析による交差性が予測される生物種
Full Name itchy E3 ubiquitin protein ligase homolog (mouse)
Calculated molecular weight 903 aa, 103 kDa
Observed molecular weight103 kDa, 70 kDa
GenBank accession numberBC011571
Gene symbol ITCH
Gene ID (NCBI) 83737
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ITCH antibody CL488-67757Download protocol
FC protocol for CL Plus 488 ITCH antibody CL488-67757Download protocol
Standard Protocols
Click here to view our Standard Protocols