Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 27096-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 27096-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of mouse kidney using 27096-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 27096-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 27096-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 27096-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 27096-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 27096-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 27096-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 27096-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27096-1-AP (Integrin alpha-V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 27096-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (27096-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of A549 using 27096-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (27096-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Featured Product
KD/KO Validated

Integrin Alpha V Polyclonal antibody

Integrin Alpha V Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27096-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ITGAV, CD51, Integrin alpha-V, Integrin alpha-V heavy chain, Integrin alpha-V light chain

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (17)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HUVEC cells, A549 cells, MCF-7 cells, human placenta tissue
Positive IHC detected in	mouse kidney tissue, human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 16 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

27096-1-AP targets Integrin Alpha V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Integrin Alpha V fusion protein Ag25825 相同性解析による交差性が予測される生物種
Full Name	integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
Calculated molecular weight	1048 aa, 116 kDa
Observed molecular weight	130 kDa
GenBank accession number	BC136442
Gene Symbol	Integrin alpha V
Gene ID (NCBI)	3685
RRID	AB_2880753
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P06756
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Integrin Alpha V antibody 27096-1-AP	Download protocol
IHC protocol for Integrin Alpha V antibody 27096-1-AP	Download protocol
IF protocol for Integrin Alpha V antibody 27096-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Theranostics Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2 Authors - Zirong Chen View Article
rat	WB	Aging Cell Fibronectin type III domain-containing 5 improves aging-related cardiac dysfunction in mice. Authors - Can Hu KD Validated View Article
human	WB	Curr Biol The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface. Authors - Adrien Presle View Article
human	WB,IHC	Int J Mol Sci Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression. Authors - Justin K Messex View Article
human	WB	Cell Signal C1GALT1 high expression enhances the progression of glioblastoma through the EGFR-AKT/ERK cascade Authors - Yanting Su View Article
human	WB	Int J Mol Sci Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients. Authors - Emma Plana View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 27096-1-AP

WB analysis using 27096-1-AP

IHC staining of mouse kidney using 27096-1-AP

IHC staining of mouse kidney using 27096-1-AP

IHC staining of human breast cancer using 27096-1-AP

IHC staining of human breast cancer using 27096-1-AP

IHC staining of human liver cancer using 27096-1-AP

IHC staining of human liver cancer using 27096-1-AP

IHC staining of human stomach cancer using 27096-1-AP

IHC staining of human stomach cancer using 27096-1-AP

IF Staining of A549 using 27096-1-AP

IF Staining of A549 using 27096-1-AP

Integrin Alpha V Polyclonal antibody

Integrin Alpha V Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2

Fibronectin type III domain-containing 5 improves aging-related cardiac dysfunction in mice.

The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface.

Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression.

C1GALT1 high expression enhances the progression of glioblastoma through the EGFR-AKT/ERK cascade

Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients.