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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated Integrin Alpha V Pol (CL488-27096)

    IF Staining of A549 using CL488-27096

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 Integrin Alpha V antibody (CL488-27096) at dilution of 1:200, CL594-phalloidin (red).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Integrin Alpha V Polyclonal antibody

Integrin Alpha V Polyclonal Antibody for IF/ICC
Cat No. CL488-27096

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

ITGAV, MSK8, Integrin alpha-V light chain, Integrin alpha-V heavy chain, Integrin alpha-V

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-27096 targets Integrin Alpha V in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Alpha V fusion protein Ag25825 相同性解析による交差性が予測される生物種
Full Name integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
Calculated molecular weight 1048 aa, 116 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC136442
Gene Symbol Integrin alpha V
Gene ID (NCBI) 3685
RRIDAB_3084107
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06756
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Integrin Alpha V antibody CL488-27096Download protocol
Standard Protocols
Click here to view our Standard Protocols