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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ITK Polyclonal antibody

ITK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21525-1-AP

Synonyms

Kinase EMT, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, IL2 inducible T cell kinase, EMT

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ITK Polyclonal antibody (21525-1-AP)

    WB analysis using 21525-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ITK Polyclonal antibody (21525-1-AP)

    WB analysis of mouse spleen using 21525-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ITK Polyclonal antibody (21525-1-AP)

    WB analysis of mouse spleen using 21525-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ITK Polyclonal antibody (21525-1-AP)

    WB analysis of Jurkat using 21525-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ITK Polyclonal antibody (21525-1-AP)

    IF Staining of Jurkat using 21525-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ITK antibody (21525-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21525-1-AP targets ITK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITK fusion protein Ag16132 相同性解析による交差性が予測される生物種
Full Name IL2-inducible T-cell kinase
Calculated molecular weight 620 aa, 72 kDa
Observed molecular weight 68-72 kDa
GenBank accession numberBC109077
Gene symbol ITK
Gene ID (NCBI) 3702
RRIDAB_10733884
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ITK antibody 21525-1-APDownload protocol
IF protocol for ITK antibody 21525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol

Role of Signal-Transducing Adaptor Protein-1 for T Cell Activation and Pathogenesis of Autoimmune Demyelination and Airway Inflammation

Authors - Kota Kagohashi
View Article
humanWB

Cytotherapy

Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma

Authors - Wenjing Luo
View Article