Validation Data Gallery
Filter:
Tested Applications
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-21525 targets ITK in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ITK fusion protein Ag16132 相同性解析による交差性が予測される生物種 |
| Full Name | IL2-inducible T-cell kinase |
| Calculated molecular weight | 620 aa, 72 kDa |
| Observed molecular weight | 68-72 kDa |
| GenBank accession number | BC109077 |
| Gene Symbol | ITK |
| Gene ID (NCBI) | 3702 |
| RRID | AB_2919639 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08881 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 ITK antibody CL555-21525 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |