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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IVNS1ABP Recombinant antibody (84268-1-RR)

    WB analysis using 84268-1-RR (same clone as 84268-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84268-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using IVNS1ABP Recombinant antibody (84268-1-RR)

    WB analysis of mouse ovary using 84268-1-RR (same clone as 84268-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84268-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IVNS1ABP Recombinant antibody (84268-1-RR)

    IHC staining of mouse kidney using 84268-1-RR (same clone as 84268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84268-1-RR (IVNS1ABP antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84268-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IVNS1ABP Recombinant antibody (84268-1-RR)

    IHC staining of mouse kidney using 84268-1-RR (same clone as 84268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84268-1-RR (IVNS1ABP antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84268-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IVNS1ABP Recombinant antibody (84268-1-RR)

    IF Staining of HeLa using 84268-1-RR (same clone as 84268-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IVNS1ABP antibody (84268-1-RR, Clone: 241587B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84268-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using IVNS1ABP Recombinant antibody (84268-1-RR)

    FC experiment of HeLa using 84268-1-RR (same clone as 84268-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug IVNS1ABP Recombinant antibody (84268-1-RR, Clone:241587B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84268-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01182-1

    Cytometric bead array standard curve of MP01182-1

    Cytometric bead array standard curve of MP01182-1, IVNS1ABP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84268-4-PBS. Detection antibody: 84268-1-PBS. Standard: Ag6412. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01182-2

    Cytometric bead array standard curve of MP01182-2

    Cytometric bead array standard curve of MP01182-2, IVNS1ABP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84268-2-PBS. Detection antibody: 84268-1-PBS. Standard: Ag6412. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 84268-1-RR

    Affinity and Kinetic Characterization of 84268-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84268-1-RR against Human IVNS1ABP were performed. The affinity constant is below 1 pM.

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IVNS1ABP Recombinant antibody, PBS Only (Detector)

IVNS1ABP Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84268-1-PBS
Clone No.241587B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

Influenza virus NS1A-binding protein, HSPC068, FLARA3, Aryl hydrocarbon receptor-associated protein 3, ARA3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84268-1-PBS targets IVNS1ABP as part of a matched antibody pair:

MP01182-1: 84268-4-PBS capture and 84268-1-PBS detection (validated in Cytometric bead array)

MP01182-2: 84268-2-PBS capture and 84268-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IVNS1ABP fusion protein Ag6412 相同性解析による交差性が予測される生物種
Full Name influenza virus NS1A binding protein
Calculated molecular weight 72 kDa
Observed molecular weight70 kDa
GenBank accession numberBC067739
Gene Symbol IVNS1ABP
Gene ID (NCBI) 10625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6Y0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IVNS1ABP is involved in many cell functions, including pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, F-actin organization and protein ubiquitination. It may play a role in mRNA nuclear export.