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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IVNS1ABP Recombinant antibody (84268-1-RR)

    WB analysis using 84268-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using IVNS1ABP Recombinant antibody (84268-1-RR)

    WB analysis of mouse ovary using 84268-1-RR

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IVNS1ABP Recombinant antibody (84268-1-RR)

    IHC staining of mouse kidney using 84268-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84268-1-RR (IVNS1ABP antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using IVNS1ABP Recombinant antibody (84268-1-RR)

    IHC staining of mouse kidney using 84268-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84268-1-RR (IVNS1ABP antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IVNS1ABP Recombinant antibody (84268-1-RR)

    IF Staining of HeLa using 84268-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IVNS1ABP antibody (84268-1-RR, Clone: 241587B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using IVNS1ABP Recombinant antibody (84268-1-RR)

    FC experiment of HeLa using 84268-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug IVNS1ABP Recombinant antibody (84268-1-RR, Clone:241587B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84268-1-RR

    Affinity and Kinetic Characterization of 84268-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84268-1-RR against Human IVNS1ABP were performed. The affinity constant is below 1 pM.

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IVNS1ABP Recombinant antibody

IVNS1ABP Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84268-1-RR
Clone No.241587B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Influenza virus NS1A-binding protein, HSPC068, FLARA3, Aryl hydrocarbon receptor-associated protein 3, ARA3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-251 cells, mouse ovary tissue, HEK-293 cells, HepG2 cells, HeLa cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84268-1-RR targets IVNS1ABP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IVNS1ABP fusion protein Ag6412 相同性解析による交差性が予測される生物種
Full Name influenza virus NS1A binding protein
Calculated molecular weight 72 kDa
Observed molecular weight70 kDa
GenBank accession numberBC067739
Gene Symbol IVNS1ABP
Gene ID (NCBI) 10625
RRIDAB_3671815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6Y0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IVNS1ABP is involved in many cell functions, including pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, F-actin organization and protein ubiquitination. It may play a role in mRNA nuclear export.

Protocols

Product Specific Protocols
WB protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
IHC protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
IF protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
FC protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols