FITC Rat IgG2a Isotype Control (2A3)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

2A3

Cat no : FITC-65209

Synonyms

IgG2a, IgG2a Isotype Control, Rat IgG2a Isotype Control


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Tested Applications

Positive FC detected inBALB/c mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

FITC-65209 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name IgG2a Isotype Control
Gene symbol
Gene ID (NCBI)
RRIDAB_2883823
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The 2A3 immunoglobulin is useful as an isotype-matched control. The 2A3 immunolglobulin binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for FITC IgG2a Isotype Control antibody FITC-65209Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Int Immunopharmacol

Neutrophil PPIF exacerbates lung ischemia-reperfusion injury after lung transplantation by promoting calcium overload-induced neutrophil extracellular traps formation

Authors - Wensi Wu