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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10268

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated IkB Alpha Polyclonal (CL488-10268)

    IF Staining of HeLa using CL488-10268

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 IkB Alpha antibody (CL488-10268) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated IkB Alpha Polyclonal (CL488-10268)

    IF Staining of A431 using CL488-10268

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 IkB Alpha antibody (CL488-10268) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10268 targets IkB Alpha in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IkB Alpha fusion protein Ag0345 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated molecular weight 36 kDa
GenBank accession numberBC002601
Gene symbol IkB Alpha
Gene ID (NCBI) 4792
RRIDAB_2918968
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IkB Alpha antibody CL488-10268Download protocol
Standard Protocols
Click here to view our Standard Protocols