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Importin Alpha 5 Monoclonal antibody

Importin Alpha 5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A7G7

Cat no : 67897-1-Ig

Synonyms

Importin subunit alpha 1, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    WB analysis using 67897-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67897-1-Ig (KPNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of HeLa cells using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    WB analysis of HeLa using 67897-1-Ig

    WB result of Importin Alpha 5 antibody (67897-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Alpha 5 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    WB analysis using 67897-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67897-1-Ig (KPNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    WB analysis of 4T1 using 67897-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67897-1-Ig (KPNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    IHC staining of mouse testis using 67897-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67897-1-Ig (Importin Alpha 5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    IF Staining of MCF-7 using 67897-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Importin Alpha 5 antibody (67897-1-Ig, Clone: 2A7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    IF Staining of MCF-7 using 67897-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Importin Alpha 5 antibody (67897-1-Ig, Clone: 2A7G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHCT 116 cells, HSC-T6 cells, 4T1 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67897-1-Ig targets Importin Alpha 5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Importin Alpha 5 fusion protein Ag12751 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 1 (importin alpha 5)
Calculated molecular weight 60 kDa
Observed molecular weight60-62 kDa
GenBank accession numberBC002374
Gene symbol Importin Alpha 5
Gene ID (NCBI) 3836
RRIDAB_2918653
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.

Protocols

Product Specific Protocols
WB protocol for Importin Alpha 5 antibody 67897-1-IgDownload protocol
IHC protocol for Importin Alpha 5 antibody 67897-1-IgDownload protocol
IF protocol for Importin Alpha 5 antibody 67897-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

bioRxiv

Palmitoylated Importin α Regulates Mitotic Spindle Orientation Through Interaction with NuMA

Authors - Patrick James Sutton
View Article