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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    WB analysis using 67897-1-Ig (same clone as 67897-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67897-1-Ig (KPNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    WB analysis of HeLa using 67897-1-Ig (same clone as 67897-1-PBS)

    WB result of Importin Alpha 5 antibody (67897-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Alpha 5 transfected HeLa cells. This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    WB analysis using 67897-1-Ig (same clone as 67897-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67897-1-Ig (KPNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    WB analysis of 4T1 using 67897-1-Ig (same clone as 67897-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67897-1-Ig (KPNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    IHC staining of mouse testis using 67897-1-Ig (same clone as 67897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67897-1-Ig (Importin Alpha 5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    IF Staining of MCF-7 using 67897-1-Ig (same clone as 67897-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Importin Alpha 5 antibody (67897-1-Ig, Clone: 2A7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Importin Alpha 5 Monoclonal antibody (67897-1-Ig)

    IF Staining of MCF-7 using 67897-1-Ig (same clone as 67897-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Importin Alpha 5 antibody (67897-1-Ig, Clone: 2A7G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67897-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Importin Alpha 5 Monoclonal antibody, PBS Only

Importin Alpha 5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67897-1-PBS
Clone No.2A7G7

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Importin subunit alpha 1, IPOA5, Karyopherin subunit alpha 1, KPNA1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1 beta

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67897-1-PBS targets Importin Alpha 5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Importin Alpha 5 fusion protein Ag12751 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 1 (importin alpha 5)
Calculated molecular weight 60 kDa
Observed molecular weight60-62 kDa
GenBank accession numberBC002374
Gene Symbol Importin Alpha 5
Gene ID (NCBI) 3836
RRIDAB_2918653
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52294
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.