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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Importin Beta Monoclonal antibody

Importin Beta Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A12B1

Cat no : CL488-67597

Synonyms

KPNB1, Importin subunit beta-1, Importin subunit beta 1, Importin 90, Impnb

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated Importin Beta Monocl (CL488-67597)

    FC experiment of Jurkat using CL488-67597

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Importin Beta (CL488-67597, Clone:1A12B1) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67597 targets Importin Beta in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Importin Beta fusion protein Ag23094 相同性解析による交差性が予測される生物種
Full Name karyopherin (importin) beta 1
Calculated molecular weight 97 kDa
Observed molecular weight 95-97 kDa
GenBank accession numberBC003572
Gene symbol KPNB1
Gene ID (NCBI) 3837
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Importin Beta antibody CL488-67597Download protocol
Standard Protocols
Click here to view our Standard Protocols