CoraLite® Plus 488-conjugated INS Monoclonal antibody
INS Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4B6A7
Cat no : CL488-66198
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 2 publications below |
Product Information
CL488-66198 targets INS in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | INS fusion protein Ag8630 相同性解析による交差性が予測される生物種 |
| Full Name | INS |
| Calculated molecular weight | 110 aa, 12 kDa |
| GenBank accession number | BC005255 |
| Gene symbol | INS |
| Gene ID (NCBI) | 3630 |
| RRID | AB_2883281 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. It is synthesized as a precursor molecule, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 INS antibody CL488-66198 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Endocrinology Discordant effects of polyamine depletion by DENSpm and DFMO on β cell cytokine stress and diabetes outcomes in mice | ||
bioRxiv Correlative Imaging for Comprehensive Molecular Mapping of Individual Cell Types in Biological Tissues |
