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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated Integrin Alpha 3 Mon (CL488-66070)

    WB analysis using CL488-66070

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66070 (Integrin Alpha 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Integrin Alpha 3 Monoclonal antibody

Integrin Alpha 3 Monoclonal Antibody for WB
Cat No. CL488-66070
Clone No.3F9G4

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse

Applications

WB

Synonyms

CD49C, FLJ34631, FLJ34704, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66070 targets Integrin Alpha 3 in WB applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Integrin Alpha 3 fusion protein Ag17293 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
Calculated molecular weight 1066 aa, 119 kDa
Observed molecular weight 120-140 kDa
GenBank accession numberBC150190
Gene Symbol Integrin alpha 3
Gene ID (NCBI) 3675
RRIDAB_3084202
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26006
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 Integrin Alpha 3 antibody CL488-66070Download protocol
Standard Protocols
Click here to view our Standard Protocols