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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Integrin Beta 1 Recombinant antibody, PBS Only

Integrin Beta 1 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1K20

Cat no : 82729-3-PBS

Synonyms

ITGB1, Integrin beta-1, GPIIA, Glycoprotein IIa, FNRB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin Beta 1 Recombinant antibody (82729-3-RR)

    WB analysis using 82729-3-RR (same clone as 82729-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Integrin Beta 1 Recombinant antibody (82729-3-RR)

    IHC staining of human placenta using 82729-3-RR (same clone as 82729-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Integrin Beta 1 Recombinant antibody (82729-3-RR)

    IHC staining of human placenta using 82729-3-RR (same clone as 82729-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82729-3-RR (Integrin beta-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82729-3-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82729-3-PBS targets Integrin Beta 1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Integrin Beta 1 fusion protein Ag3078 相同性解析による交差性が予測される生物種
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated molecular weight 88 kDa
Observed molecular weight110-140 kDa
GenBank accession numberBC020057
Gene symbol Integrin beta 1
Gene ID (NCBI) 3688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.