Integrin Beta 1 Recombinant antibody
Integrin Beta 1 Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1K20
Cat no : 82729-3-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-1080 cells, HeLa cells, A431 cells, U-87 MG cells, HCT 116 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82729-3-RR targets Integrin Beta 1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Integrin Beta 1 fusion protein Ag3078 相同性解析による交差性が予測される生物種 |
| Full Name | integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) |
| Calculated molecular weight | 88 kDa |
| Observed molecular weight | 110-140 kDa |
| GenBank accession number | BC020057 |
| Gene symbol | Integrin beta 1 |
| Gene ID (NCBI) | 3688 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Beta 1 antibody 82729-3-RR | Download protocol |
| IHC protocol for Integrin Beta 1 antibody 82729-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
