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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Involucrin Recombinant antibody, PBS Only

Involucrin Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240566D8

Cat no : 83649-5-PBS

Synonyms

IVL, 240566D8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Involucrin Recombinant antibody (83649-5-RR)

    WB analysis using 83649-5-RR (same clone as 83649-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83649-5-RR (Involucrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83649-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using Involucrin Recombinant antibody (83649-5-RR)

    IHC staining of human skin using 83649-5-RR (same clone as 83649-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83649-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Involucrin Recombinant antibody (83649-5-RR)

    IHC staining of human skin cancer using 83649-5-RR (same clone as 83649-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83649-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83649-5-RR

    Affinity and Kinetic Characterization of 83649-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83649-5-RR against Human Involucrin were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83649-5-PBS is Involucrin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Involucrin fusion protein Ag29585 相同性解析による交差性が予測される生物種
Full Name involucrin
Calculated molecular weight 68 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_005547
Gene symbol Involucrin
Gene ID (NCBI) 3713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)