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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Involucrin Recombinant antibody (83649-5-RR)

    WB analysis using 83649-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83649-5-RR (Involucrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using Involucrin Recombinant antibody (83649-5-RR)

    IHC staining of human skin using 83649-5-RR

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Involucrin Recombinant antibody (83649-5-RR)

    IHC staining of human skin cancer using 83649-5-RR

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83649-5-RR

    Affinity and Kinetic Characterization of 83649-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83649-5-RR against Human Involucrin were performed. The affinity constant is below 1 pM.

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Involucrin Recombinant antibody

Involucrin Recombinant Antibody for WB, IHC, ELISA
Cat No. 83649-5-RR
Clone No.240566D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

IVL, 240566D8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HaCaT cells, HT-29 cells, HT-1080 cells
Positive IHC detected inhuman skin tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83649-5-RR targets Involucrin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Involucrin fusion protein Ag29585 相同性解析による交差性が予測される生物種
Full Name involucrin
Calculated molecular weight 68 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_005547
Gene Symbol Involucrin
Gene ID (NCBI) 3713
RRIDAB_3671258
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP07476
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)

Protocols

Product Specific Protocols
WB protocol for Involucrin antibody 83649-5-RRDownload protocol
IHC protocol for Involucrin antibody 83649-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols