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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Islet 1 Polyclonal antibody

Islet 1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15661

Synonyms

ISL1, Islet-1, ISLET1, ISL LIM homeobox 1, Isl 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated Islet 1 Polyclonal a (CL488-15661)

    IF Staining of SH-SY5Y using CL488-15661

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 Islet 1 antibody (CL488-15661) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 488-conjugated Islet 1 Polyclonal a (CL488-15661)

    FC experiment of SH-SY5Y using CL488-15661

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Islet 1 (CL488-15661) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15661 targets Islet 1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Islet 1 fusion protein Ag8088 相同性解析による交差性が予測される生物種
Full Name ISL LIM homeobox 1
Calculated molecular weight 39 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberBC031213
Gene symbol Islet 1
Gene ID (NCBI) 3670
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Islet 1 antibody CL488-15661Download protocol
FC protocol for CL Plus 488 Islet 1 antibody CL488-15661Download protocol
Standard Protocols
Click here to view our Standard Protocols