Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-83784-4 targets Islet 1 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Islet 1 fusion protein Ag33541 相同性解析による交差性が予測される生物種 |
| Full Name | ISL LIM homeobox 1 |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 39-45 kDa |
| GenBank accession number | BC031213 |
| Gene Symbol | Islet 1 |
| Gene ID (NCBI) | 3670 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P61371 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Islet 1 antibody CL488-83784-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |