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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using JMJD4 Polyclonal antibody (21572-1-AP)

    WB analysis of HeLa using 21572-1-AP (same clone as 21572-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21572-1-AP (JMJD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21572-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using JMJD4 Polyclonal antibody (21572-1-AP)

    WB analysis of HEK-293 using 21572-1-AP (same clone as 21572-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21572-1-AP (JMJD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21572-1-PBS in a different storage buffer formulation.

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JMJD4 Polyclonal antibody, PBS Only

JMJD4 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 21572-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21572-1-PBS targets JMJD4 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JMJD4 fusion protein Ag16099 相同性解析による交差性が予測される生物種
Full Name jumonji domain containing 4
Calculated molecular weight 463 aa, 52 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC109272
Gene Symbol JMJD4
Gene ID (NCBI) 65094
RRIDAB_2878886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9V9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

JMJD4 (JmjC domain-containing protein 4, Jumonji domain-containing protein 4) is a member of the JmjC domain-containing proteins. Several JmjC domain-containing proteins have been reported to play a crucial role in the control of gene expression by acting as protein hydroxylases or histone demethylases (PMID: 16983801; 21300889). The function of JMJD4 protein remains unknown. The gene of JMJD4 maps to chromosome 1q42.13.