Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HT-29 cells |
Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 12 publications below |
| FC | See 1 publications below |
Product Information
66890-1-Ig targets Jagged1 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, goat |
| Host / Isotype | Mouse / IgA |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Jagged1 fusion protein Ag11891 相同性解析による交差性が予測される生物種 |
| Full Name | jagged 1 (Alagille syndrome) |
| Calculated molecular weight | 1218 aa, 134 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC098393 |
| Gene Symbol | Jagged1 |
| Gene ID (NCBI) | 182 |
| RRID | AB_2882220 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Thiophilic affinity chromatograph |
| UNIPROT ID | P78504 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Jagged1 is a type I, membrane- anchored, multidomain protein and a ligand of the Notch receptor. The receptor-ligand interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis. The predicted MW of Jagged1 is 134 kDa, while higher MW around 150-180 kDa had been observed in literations (PMID: 9716576, 9596653).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Jagged1 antibody 66890-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Oncol Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis | ||
Int J Biol Sci Linc-KILH potentiates Notch1 signaling through inhibiting KRT19 phosphorylation and promotes the malignancy of hepatocellular carcinoma. | ||
Commun Biol Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells. | ||
Front Med (Lausanne) IL-7-Treated Periodontal Ligament Cells Regulate Local Immune Homeostasis by Modulating Treg/Th17 Cell Polarization. | ||
Cell Biochem Funct LncRNA TDRG1/miR-214-5p axis affects preeclampsia by modulating trophoblast cells. | ||
Acta Histochem miR-214-5p suppresses the proliferation, migration and invasion of trophoblast cells in pre-eclampsia by targeting jagged 1 to inhibit notch signaling pathway. |