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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Jagged1 Monoclonal antibody (66890-1-Ig)

    WB analysis of HEK-293 using 66890-1-Ig (same clone as 66890-1-PBS)

    WB result of Jagged1 antibody (66890-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Jagged1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66890-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using Jagged1 Monoclonal antibody (66890-1-Ig)

    WB analysis of HT-29 using 66890-1-Ig (same clone as 66890-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66890-1-Ig (Jagged1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66890-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Jagged1 Monoclonal antibody, PBS Only

Jagged1 Monoclonal Antibody for WB, ELISA
Cat No. 66890-1-PBS
Clone No.2A7D11

Host / Isotype

Mouse / IgA

Reactivity

Human

Applications

WB, ELISA

Synonyms

AGS, AHD, AWS, CD339, HJ1, JAG1, jagged 1 (Alagille syndrome), Jagged1, JAGL1, Protein jagged 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66890-1-PBS targets Jagged1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen Jagged1 fusion protein Ag11891 相同性解析による交差性が予測される生物種
Full Name jagged 1 (Alagille syndrome)
Calculated molecular weight 1218 aa, 134 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC098393
Gene Symbol Jagged1
Gene ID (NCBI) 182
RRIDAB_2882220
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78504
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Jagged1 is a type I, membrane- anchored, multidomain protein and a ligand of the Notch receptor. The receptor-ligand interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis. The predicted MW of Jagged1 is 134 kDa, while higher MW around 150-180 kDa had been observed in literations (PMID: 9716576, 9596653).